Microscope system

ABSTRACT

A plurality of mounting devices are selectively exchanged while keeping the same parfocal distances for objective lenses among these mounting devices. A microscope system includes a microscope main unit and a plurality of attachable/detachable objective lens units that are selectively attached to the microscope main unit. The microscope main unit includes raising mechanism that can move the attached objective lens unit in an optical axis direction. The plurality of objective lens units have a revolver or a nosepiece that can be attached to the microscope main unit and an objective lens that can be mounted on the revolver or the nosepieces in an attachable/detachable manner. Distances from an attachment position in the microscope main unit for the revolver or the nosepiece to focal positions of the objective lenses are set to be mutually equal among the objective lens units.

This application is based on Japanese Patent Application No.2011-159301, the contents of which are incorporated herein by reference.

TECHNICAL FIELD

The present invention relates to a microscope system.

BACKGROUND ART

In the related art, there is a microscope device that has a mountingdevice that is provided with a plurality of objective lenses in anattachable/detachable manner and that can selectively place theseobjective lenses in an observation optical path of the microscope (forexample, see Patent Literature 1). The microscope device disclosed inPatent Literature 1 is provided with a mounting device that has a firstholding member that holds a first objective lens; a second holdingmember that holds a second objective lens having a shorter workingdistance than the first objective lens; a raising mechanism that movesthe second objective lens in an optical axis direction; and an adjustingmechanism that adjusts the position of the second objective lens in theoptical axis direction moved by the raising mechanism.

With this microscope device disclosed in Patent Literature 1, a state inwhich the first objective lens having a longer working distance isplaced in the observation optical path of the microscope is used as areference, and the first objective lens is switched to the secondobjective lens while maintaining parfocal distances therefor by movingthe second objective lens in the optical axis direction with the raisingmechanism and by adjusting the position to which the second objectivelens is moved with the adjusting mechanism.

CITATION LIST Patent Literature {PTL 1}

Publication of Japanese Patent No. 3900664

SUMMARY OF INVENTION Technical Problem

However, in the case in which multiple types of objective lenses withdifferent parfocal distances are used by switching them, the wholemounting device is exchanged in some cases. In such a case, the mountingdevice is exchanged with another mounting device in a state in which itis temporarily withdrawn from a specimen on a stage in the optical axisdirection in order to ensure enough space; however, because thewithdrawal distance in the optical axis direction differs for eachmounting device, there is a problem in that, even if the new mountingdevice, after exchange, is moved back by the same distance by which themounting device before exchange is withdrawn, the image may be out offocus or the objective lens may end up coming into contact with thespecimen.

The present invention has been conceived in light of the above-describedcircumstances, and an object thereof is to provide a microscope systemin which a plurality of mounting devices can be employed by selectivelyexchanging them while maintaining parfocal distances for objectivelenses among the plurality of mounting devices.

Solution to Problem

In order to achieve the above-described object, the present inventionemploys the following solutions.

An aspect of the present invention is a microscope system including amicroscope main unit that generates illumination light to be radiatedonto a specimen and that detects detection light from the specimen; anda plurality of attachable/detachable objective lens units that areselectively attached to the microscope main unit, the microscope mainunit including a raising mechanism that can move the attached objectivelens unit in an optical axis direction; wherein the plurality ofobjective lens units have a mounting device that can be attached to theraising mechanism and an objective lens that is mounted on the mountingdevice in an attachable/detachable manner, and that collects thedetection light from the specimen; and distances from a mountingposition in the microscope main unit for the mounting device to focalpositions of the objective lenses are set to be mutually equal amongthese objective lens units.

With this aspect, the plurality of objective lens units are employed bybeing selectively attached to the microscope main unit, and themicroscope main unit detects the detection light from the specimenthrough the objective lens of one objective lens unit attached thereto.

In this case, by setting the distances from the mounting position in themicroscope main unit for the mounting device to the focal positions ofthe objective lenses to be mutually equal among the objective lensunits, even if the objective lens unit is moved in the optical axisdirection with the raising mechanism in order to ensure sufficient spacewhen exchanging the objective lens unit, the distance from the mountingposition in the mounting device of the objective lens unit for theobjective lens to the focal position of the objective lens (the distancefrom the mounting position in the mounting device for the objective lensto the focal position of the objective lens will be hereinafter referredto as “parfocal distance”) before exchange and the parfocal distance forthe objective lens unit after exchange can be maintained in a simplemanner. Therefore, the plurality of objective lens units can be employedby selectively exchanging them while maintaining the parfocal distancesfor the objective lenses among the plurality of the objective lensunits.

In the above-described aspect, the mounting device of one of theobjective lens units may have a mounting device main unit to which theobjective lens is mounted and a mounting adaptor that is fixed to themounting device main unit and that attaches the mounting device mainunit to the microscope main unit in an attachable/detachable manner.

With such a configuration, even if the size of the mounting device mainunit of one of the objective lens units is smaller than the sizes ofmounting devices of the other objective lens units formed as a singlepiece, the size difference is compensated for with the mounting adaptor,and the distances from the attachment positions where the mountingdevices are attached to the microscope main unit to the focal positionsof the objective lenses can be mutually matched among these objectivelens units.

In the above-described aspect, the objective lens unit includes a firstobjective lens and a second objective lens, wherein a distance in theoptical axis direction from an end on a mounting device side of thefirst objective lens to a focal position of the first objective lens isset to be shorter than a distance in the optical axis direction from anend on a mounting device side of the second objective lens to a focalposition of the second objective lens; and a distance in the opticalaxis direction from a mounting position for the first objective lens inthe mounting device to the focal position of the first objective lens isset to be equal to a distance in the optical axis direction from themounting position for the second objective lens in the mounting deviceto the focal position of the second objective lens.

In the above-described aspect, an objective lens adaptor interposedbetween the second objective lens and the mounting device.

Advantageous Effects of Invention

The present invention affords an advantage in that a plurality ofmounting devices can be employed by selectively exchanging them whilemaintaining parfocal distances for objective lenses among the pluralityof mounting devices.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 is a schematic configuration diagram showing a microscope systemaccording to an embodiment of the present invention.

FIG. 2 is a schematic configuration diagram showing an imaging objectivelens unit.

FIG. 3 is a schematic configuration diagram showing a patch-clamping orin-vivo objective lens unit.

FIG. 4 is a schematic configuration diagram showing anotherpatch-clamping or in-vivo objective lens unit.

FIG. 5 is a schematic configuration diagram showing anotherpatch-clamping or in-vivo objective lens unit.

FIG. 6 is a schematic configuration diagram showing a case in which aspecimen is observed by attaching the imaging objective lens unit to amicroscope main unit.

FIG. 7 is a schematic configuration diagram showing a case in which aspecimen is observed by attaching the patch-clamping or in-vivoobjective lens unit.

FIG. 8 is a schematic configuration diagram showing a case in which aspecimen is observed by attaching another patch-clamping or in-vivoobjective lens unit.

FIG. 9 is a schematic configuration diagram showing a case in which aspecimen is observed by attaching another patch-clamping or in-vivoobjective lens unit.

DESCRIPTION OF EMBODIMENT

A microscope system according to an embodiment of the present inventionwill be described below with reference to the drawings.

As shown in FIG. 1, a microscope system 100 according to this embodimentis provided with, for example, a microscope main unit 10 and a pluralityof attachable/detachable objective lens units 20, 30, 40, and 50 thatare selectively attached to the microscope main unit 10.

The microscope main unit 10 is provided with a stage 11 on which aspecimen (not shown) is placed; a light source (not shown) that emitsillumination light for incident-light illumination; a condenser 13 thatirradiates the specimen with illumination light emitted from a lightsource (not shown) for transmission illumination; an arm 15 to which theobjective lens unit 20, 30, 40, or 50 is selectively attached; ailluminator 17 that has an illumination optical system (not shown)inside thereof; a frame 19 that supports the condenser 13, the arm 15,and the illuminator 17; and a detection unit (not shown) that detectsdetection light returning from the specimen irradiated with theillumination light.

This microscope main unit 10 is an upright microscope with a fixed stagewhere focusing is performed by moving the objective lens unit 20, 30,40, or 50 up and down with respect to the stage 11. The microscope mainunit 10 is provided with, for example, an electrically powered raisingmechanism 16 with which the attached objective lens unit 20, 30, 40, or50 can be moved in an optical axis direction.

The frame 19 is formed in a L-shape with a base portion 19 a in thehorizontal direction and a body portion 19 b formed perpendicular to thebase portion 19 a.

The stage 11 is supported by a stage-supporting post (not shown) and isplaced above the base portion 19 a of the frame 19 so that it can be setat a predetermined height. In addition, the stage 11 is provided so thatit can be moved in the horizontal directions (XY-axial directions)perpendicular to the height direction (Z-axial direction) in the statein which it is supported by the stage-supporting post.

The condenser 13 is supported by a condenser holder 14 that is providedin an attachable/detachable manner on the base portion 19 a of the frame19 so as to be placed facing the objective lens unit 20, 30, 40, or 50with the stage 11 interposed therebetween. This condenser 13 is providedfor the condenser holder 14 in an attachable/detachable manner so thatit can be directly installed on the base portion 19 a of the frame 19.

The arm 15 is provided in an attachable/detachable manner at astage-11-side wall surface of the body portion 19 b of the frame 19 andis provided so that it can be moved up and down along the stage-11-sidewall surface with the raising mechanism 16. In FIG. 1, reference sign Dindicates the stroke of the arm 15.

This arm 15 holds the objective lens unit 20, 30, 40 or 50 in anattachable/detachable manner so that the objective lens unit 20, 30, 40,or 50 is placed above the stage 11. In FIG. 1, reference sign 15 aindicates an attachment position in the arm 15 for the objective lensunit 20, 30, 40, or 50.

The illuminator 17 is provided at the top end of the body portion 19 bof the frame 19 and is placed so as to protrude above the stage 11. Aheight-increasing member 18 having a predetermined thickness is providedin an attachable/detachable manner between the illuminator 17 and thetop end of the body portion 19 b of the frame 19. By attaching/detachingthe height-increasing member 18, a reference position of the arm 15 andthe height of the illuminator 17 can be adjusted.

The objective lens units 20, 30, 40, and 50 may be, for example, animaging objective lens unit 20, such as one shown in FIG. 2, objectivelens units 30, 40, and 50 and so on for patch-clamping or for observinga live organism (in vivo), such as those shown in FIGS. 3, 4, and 5.

As shown in FIG. 2, the imaging objective lens unit 20 is formed of arevolver (mounting device) 21 which can be attached to the arm 15 of themicroscope main unit 10 and an objective lens 23 that can be attached toand detached from the revolver 21.

The revolver 21 can be moved in the optical axis direction (Z-axialdirection) of the objective lens 23 through the up-down motion of thearm 15. In addition, the revolver 21 can be rotated about apredetermined rotation axis (not shown), and a plurality of objectivelenses 23 can be simultaneously mounted thereon in the circumferentialdirection with spaces therebetween. In FIG. 2, one objective lens 23 ismounted on the revolver 21. This revolver 21 is configured such that, byrotating it about the rotation axis, any one of the plurality ofobjective lenses 23 can selectively be placed at a position facing thespecimen.

In addition, the distances from an attachment position (mountingposition) 21 a where the revolver 21 is attached to the arm 15 tomounting positions 21 b where the objective lenses 23 are mounted on therevolver 21 are set to be dimension C. Respective lengths of theplurality of objective lenses 23 mounted on the revolver 21 are set sothat distances in the optical axis direction from mounting positions 21b in the revolver 21 for the individual objective lenses 23 to focalpositions of the individual objective lenses 23 (the distances in theoptical axis direction from the mounting positions 21 b in the revolver21 for the objective lenses 23 to the focal positions of the objectivelenses 23 will be, hereinafter, referred to as “parfocal distances”) aredimension E.

In addition, the revolver 21 is compatible with differentialinterference contrast (DIC) observation, and is provided with a DICslider 1 in an attachable/detachable manner, which serves as adifferential-interference-contrast prism unit employed in thedifferential interference contrastobservation. The DIC slider 1 ishorizontally mounted on the revolver 21 so that a DIC prism (not shown)provided in the DIC slider 1 as a differential-interference-contrastprism is placed near a pupil position of the objective lens 23.

The patch-clamping or in-vivo objective lens units 30, 40, and 50 are asfollows.

As shown in FIG. 3, the objective lens unit 30 is formed of a nosepiece(mounting device) 31 that can be attached to the arm 15 of themicroscope main unit 10 and an objective lens 33 that can be attached toand detached from the nosepiece 31.

The nosepiece 31 is provided with a swing unit 35 that can swing about apredetermined swing axis extending in the X or Y axial direction. Aplurality of objective lenses 33 can be simultaneously mounted on theswing unit 35 with spaces therebetween in the swinging direction. InFIG. 3, two objective lenses 33 are mounted on the nosepiece 31. Thisnosepiece 31 is configured such that, by swinging the swing unit 35about the swing axis, any one of the plurality of objective lenses 33can be selectively placed in a position facing the specimen.

In addition, distances from an attachment position (mounting position)31 a where the nosepiece 31 is attached to the arm 15 to mountingpositions 31 b where the objective lenses 33 are mounted on thenosepiece 31 are set to be dimension C. Respective lengths of theplurality of objective lenses 33 mounted on the nosepiece 31 are set sothat distances in the optical axis direction from the mounting positions31 b in the nosepiece 31 for the individual objective lenses 33 to focalpositions of the individual objective lenses 33 (the distances in theoptical axis direction from the mounting positions 31 b in the nosepiece31 for the objective lenses 33 to the focal positions of the objectivelenses 33 will be, hereinafter, referred to as “parfocal distances”) aredimension E.

Next, as shown in FIG. 4, the objective lens unit 40 is formed of asliding nosepiece (mounting device) 41 that can be attached to the arm15 of the microscope main unit 10 and an objective lens 43 that can beattached to and detached from the nosepiece 41. The nosepiece 41 can beslid in the X or Y axial direction and a plurality of objective lenses43 can be simultaneously mounted thereon with spaces therebetween in thesliding direction. This nosepiece 41 is configured so that, by slidingit, any one of the plurality of objective lenses 43 can be alternativelyplaced in a position facing the specimen.

In addition, distances from an attachment position (mounting position)41 a where the nosepiece 41 is attached to the arm 15 to mountingpositions 41 b where the objective lenses 43 are mounted on thenosepiece 41 are set to be dimension C. In FIG. 4, two objective lenses43 are mounted on the nosepiece 41. One of the objective lenses 43 isprovided with an objective lens adaptor 45 placed between the objectivelens 43 and the mounting position 41 b in the nosepiece 41. For one ofthe objective lenses 43, the parfocal distance thereof can be matchedwith that of the other objective lens 43 with a simple configuration inwhich the objective lens adaptor 45 is merely interposed between theobjective lens 43 and the mounting position 41 b in the nosepiece 41.

By doing so, the length of one of the objective lenses 43 is adjusted sothat the respective distances in the optical axis direction from themounting positions 41 b in the nose piece 41 for the two objectivelenses 43 to the focal positions of the individual objective lenses 43(the distances in the optical axis direction from the mounting positions41 b in the nosepiece 41 for the objective lenses 43 to the focalpositions of the objective lenses 43 will be, hereinafter, referred toas “parfocal distances”) are dimension E.

Next, as shown in FIG. 5, the objective lens unit 50 is formed of anosepiece (mounting device) 51 that can be attached to the arm 15 of themicroscope main unit 10 and an objective lens 53 that can be attached toand detached from the nosepiece 51. The nosepiece 51 has a nosepiecemain unit (mounting device main unit) 55A on which the objective lens 53is mounted and a mounting adaptor 55B that is fixed to the nosepiecemain unit 55A and that attaches the nosepiece main unit 55A to theattachment position 15 a in the arm 15 in an attachable/detachablemanner.

The nosepiece main unit 55A can be rotated about a predeterminedrotation axis (not shown) and a plurality of the objective lenses 53 canbe simultaneously mounted thereon with spaces therebetween in thecircumferential direction. In FIG. 5, one objective lens 53 is mountedon the nosepiece main unit 55A. This nosepiece 51 is configured suchthat, by rotating the nosepiece main unit 55A about the rotation axis,any one of the plurality of objective lenses 53 can be selectivelyplaced at a position facing the specimen.

This nosepiece main unit 55A has a smaller thickness A than thethickness of the nosepiece 31 or the nosepiece 41. The mounting adaptor55B has a thickness B corresponding to a difference between thethickness of the nosepiece main unit 55A and the thickness of thenosepiece 31 as well as that of the nosepiece 41. By doing so, distancesfrom an attachment position 51 a where the nosepiece 51 is attached tothe arm 15 to mounting positions 51 b where the objective lenses 53 aremounted on the nosepiece 51 are set to be dimension C. Therefore, byinterposing the mounting adaptor 55B between the attachment position(mounting position) 15 a in the arm 15 and the nosepiece main unit 55A,the nosepiece 51 compensates for size differences relative to thenosepieces 31 and 41, which are formed as single pieces.

The respective lengths of the plurality of objective lenses 53 mountedon the nosepiece 51 are set so that distances in the optical axisdirection from the mounting positions 51 b in the nosepiece 51 for theindividual objective lenses 53 to focal positions of the individualobjective lenses 53 (the distances in the optical axis direction fromthe mounting positions 51 b in the nosepiece 51 for the objective lenses53 to the focal positions of the objective lenses 53 will be,hereinafter, referred to as “parfocal distances”) are dimension E.

These patch-clamping or in-vivo objective lens units 30, 40, and 50 canalso be moved in the optical axis direction (Z direction) of theindividual objective lenses 33, 43, and 53 through the up-down movementof the arm 15. In addition, the objective lens units 30, 40, and 50 arealso compatible with differential interference contrast observation,and, as with the revolver 21, the individual nosepieces 31, 41, and 51are provided with the DIC slider 1 in an attachable/detachable manner.

In all of the above-described objective lens units 20, 30, 40, and 50,distances in the optical axis direction from the respective attachmentpositions 21 a, 31 a, 41 a, and 51 a where the revolver 21 or nosepieces31, 41, and 51 are attached to the arm 15 of the microscope main unit 10to the focal positions of the individual objective lenses 23, 33, 43 and53, that is, distances in the optical axis direction from the attachmentposition 15 a in the arm 15 to the focal positions of the individualobjective lenses 23 33, 43, and 53, are set to be the same dimension(dimension C +dimension E).

Next, the effects of the thus-configured microscope system 100 will bedescribed.

In the case in which a specimen, such as cells or the like, held on aslide glass or in a dish is observed with the microscope system 100according to this embodiment by employing the imaging objective lensunit 20, as shown in FIG. 6, the height-increasing member 18 is removedand the condenser 13 is placed above the base portion 19 a of the frame19 without using the condenser holder 14.

In addition, the height of the stage 11 is set so that the distancebetween the stage 11 and the condenser 13 is β. Furthermore, theobjective lens unit 20 is attached to the microscope main unit 10, andone of the objective lenses 23 is placed directly above the specimen bymeans of the revolver 21. The distance from the tip of the objectivelens 23 to the stage 11 when the arm 15 is at the reference position isdefined as α.

In this state, illumination light is generated at the light source fortransmission illumination, and the illumination light is radiated ontothe specimen with the condenser 13. By radiating the illumination lightfrom below the stage 11, transmission light (detection light) thatpasses through the specimen is collected by the objective lens 23 and isdetected by the detection unit. By doing so, the specimen can beobserved.

In this case, because the distances in the optical axis direction fromthe mounting positions 21 b in the revolver 21 for the plurality ofobjective lenses 23 mounted on the objective lens unit 20 to their focalpositions are set to be equal, within the objective lens unit 20, anobjective lens 23 can be easily switched to another objective lens 23having a different magnification while keeping the same parfocaldistance.

Next, in the case in which a specimen, such as cells or the like, heldon a slide glass or in a dish is observed by exchanging the imagingobjective lens unit 20 with the patch-clamping or the in-vivo objectivelens unit 30, 40, or 50, as shown in FIG. 7, the height-increasingmember 18 is mounted thereon to increase the height of the referenceposition of the arm 15 and the illuminator 17. In addition, thecondenser holder 14 is provided on the base portion 19 a of the frame 19to support the condenser 13 with the condenser holder 14. Additionally,the height of the stage 11 is set so that the distance between the stage11 and the condenser 13 is β.

For example, assuming that the objective lens unit 30 is employed, thearm 15 is moved away from the stage 11, the objective lens unit 20 isremoved, and the objective lens unit 30 is attached to the arm 15. Then,one of the objective lenses 33 is placed directly above the specimen bymeans of the nosepiece 31. By increasing the height of the arm 15 bymounting the height-increasing member 18, the distance from the tip ofthe objective lens 33 to the stage 11 when the arm 15 is at thereference position is α, even though the condenser holder 14 isprovided.

In this case, because the distances in the optical axis direction fromthe attachment position 21 a where the revolver 21 is attached to thearm 15 to the focal positions of the objective lenses 23 and thedistances in the optical axis direction from the attachment position 31a where the nosepiece 31 is attached to the arm 15 to the focalpositions of the objective lenses 33 are set to be equal, even if theobjective lens unit 20 is moved in the optical axis direction with theraising mechanism 16 in order to ensure sufficient space when exchangingthe objective lens units 20 and 30, the parfocal distance for theobjective lens unit 20 before exchange and the parfocal distance for theobjective lens unit 30 after exchange can be maintained in a simplemanner.

In the state in which one of the objective lenses 33 is placed directlyabove the specimen, the illumination light is generated at the lightsource for transmission illumination, the illumination light is radiatedonto the specimen with the condenser 13, and transmission light thatpasses through the specimen is collected by the objective lens 33 to bedetected by the detection unit. By doing so, the specimen can beobserved. Although the case in which the objective lens unit 30 isemployed is described as an example, the operation is the same for thecase in which the objective lens unit 40 or 50 is employed.

In addition, because the distances in the optical axis direction fromthe mounting positions 31 b in the nosepiece 31 for the plurality ofobjective lenses 33 mounted on the objective lens unit 30 to their focalpositions are also set to be equal in the objective lens unit 30, withinthe objective lens unit 30, an objective lens 33 can be easily switchedto another objective lens 33 having a different magnification whilekeeping the same parfocal distance.

Next, in the case in which a small animal whose size is comparativelysmall is observed as a specimen instead of a specimen such as cells orthe like held on a slide glass or in a dish by employing thepatch-clamping or the in-vivo objective lens unit 30, 40, or 50, asshown in FIG. 8, the condenser holder 14 and the condenser 13 areremoved from the base portion 19 a of the frame 19 while leaving theheight-increasing member 18 mounted in place.

Then, for example, assuming that the objective lens unit 40 is employedinstead of the objective lens unit 30, the arm 15 is moved away from thestage 11, the objective lens unit 30 is removed, and the objective lensunit 40 is attached to the arm 15. One of the objective lenses 43 isplaced directly above the specimen by means of the nosepiece 41. Inorder to ensure sufficient space between the stage 11 and the objectivelens 43 in accordance with the size of the specimen, the height of thestage 11 is set so that the distance between the stage 11 and the tip ofthe objective lens 43 is, for example, α+40 mm.

Because the distances in the optical axis direction from the attachmentposition 31 a where the nosepiece 31 is attached to the arm 15 to thefocal positions of the objective lenses 33 and the distances in theoptical axis direction from the attachment position 41 a where thenosepiece 41 is attached to the arm 15 to the focal positions of theobjective lenses 43 are similarly set to be equal in this case, theparfocal distance for the objective lens unit 30 before exchange and theparfocal distance for the objective lens unit 40 after exchange can bemaintained in a simple manner.

In the state in which one of the objective lenses 43 is placed directlyabove the specimen, illumination light is generated at the light sourcefor incident-light illumination, and the illumination light is radiatedonto the specimen with the objective lens 43 via the illuminationoptical system in the illuminator 17. Returning light (detection light)that returns from the specimen by irradiating it with the illuminationlight is collected by the objective lens 43 to be detected by thedetection unit. By doing so, the specimen can be observed. Although thecase in which the objective lens unit 40 is employed is described as anexample, the operation is the same for the case in which the objectivelens unit 30 or 50 is employed.

In addition, because the distances in the optical axis direction fromthe mounting positions 41 b in the nosepiece 41 for the plurality ofobjective lenses 43 mounted on the objective lens unit 40 to their focalpositions are also set to be equal in the objective lens unit 40, withinthe objective lens unit 40, an objective lens 43 can be easily switchedto another objective lens 43 having a different magnification whilekeeping the same parfocal distance.

Next, in the case in which a small animal whose size is comparativelylarge is observed as a specimen instead of a small animal whose size iscomparatively small by employing the patch-clamping or the in-vivoobjective lens unit 30, 40, or 50, as shown in FIG. 9, theheight-increasing member 18 is left mounted in place, the condenserholder 14 and the condenser 13 are left removed, and the height of thestage 11 is lowered in accordance with the size of the specimen.

For example, assuming that the objective lens unit 50 is employed to asto exchange the objective lens unit 40, the arm 15 is moved away fromthe stage 11, the objective lens unit 40 is removed, and the objectivelens unit 50 is attached to the arm 15. Then, one of the objectivelenses 53 is placed directly above the specimen by means of thenosepiece 51. In order to ensure sufficient space between the stage 11and the objective lens 53 in accordance with the size of the specimen,the height of the stage 11 is set so that the distance between the stage11 and the tip of the objective lens 53 is α+(40+50) mm.

Because the distances in the optical axis direction from the attachmentposition 41 a where the nosepiece 41 is attached to the arm 15 to thefocal positions of the objective lenses 43 and the distances in theoptical axis direction from the attachment position 51 a where thenosepiece 51 is attached the arm 15 to the focal positions of theobjective lenses 53 are also set to be equal in this case, the parfocaldistance for the objective lens unit 40 before exchange and the parfocaldistance for the objective lens unit 50 after exchange can be maintainedin a simple manner.

In the state in which one of the objective lenses 53 is placed directlyabove the specimen, the illumination light is generated at the lightsource for incident-light illumination to be radiated onto the specimenwith the objective lens 53 via the illumination optical system in theilluminator 17, and returning light that returns from the specimen iscollected by the objective lens 53 to be detected by the detection unit.By doing so, the specimen can be observed. Although the case in whichthe objective lens unit 50 is employed is described as an example, theoperation is the same for the case in which the objective lens unit 30or 40 is employed.

In addition, because the distances in the optical axis direction fromthe mounting positions 51 b in the nosepiece 51 for the plurality ofobjective lenses 53 mounted on the objective lens unit 50 to their focalpositions are also set to be equal in the objective lens unit 50, withinthe objective lens unit 50, an objective lens 53 can be easily switchedto another objective lens 53 having a different magnification whilekeeping the same parfocal distance.

As has been described above, with the microscope system 100 according tothis embodiment, by setting the distances in the optical axis directionfrom the respective attachment positions 21 a, 31 a, 41 a and 51 a wherethe revolver 21 or the nosepieces 31, 41, and 51 are attached to themicroscope main unit 10 to the focal positions of the individualobjective lenses 23, 33, 43, and 53 to be mutually equal among theobjective lens units 20, 30, 40, and 50, the parfocal distance for theobjective lens unit 20, 30, 40, or 50 before exchange and the parfocaldistance for the objective lens unit 20, 30, 40, or 50 after exchangecan be maintained in a simple manner when exchanging the objective lensunits 20, 30, 40, and 50, even though the objective lens unit 20, 30,40, or 50 is moved in the optical axis direction with the raisingmechanism 16 in order to ensure sufficient space. Therefore, whilemaintaining the parfocal distances for the objective lenses 23, 33, 43,and 53 among the plurality of objective lens units 20, 30, 40, and 50,these objective lens units 20, 30, 40, and 50 can be employed byselectively exchanging them.

Although the objective lens 43 is provided with the objective lensadaptor 45 in this embodiment, the other objective lens 23, 33, or 53may be provided with the objective lens adaptor, and thereby, thelengths thereof may be adjusted so that the parfocal distances of theplurality of objective lenses 23, 33, or 53 in the same objective lensunit 20, 30, or 50 are matched.

In addition, although the nosepiece 51 of the objective lens unit 50 isprovided with the mounting adaptor 55 in this embodiment, one of therevolver 21 of the objective lens unit 20, the nosepiece 31 of theobjective lens unit 30, and the nosepiece 41 of the objective lens unit40 may be provided with the mounting adaptor.

REFERENCE SIGNS LIST

10 microscope main unit

20, 30, 40, 50 objective lens unit

21 revolver (mounting device)

21 a attachment position

23 objective lens

31 nosepiece (mounting device)

31 a attachment position

33 objective lens

41 nosepiece (mounting device)

41 a attachment position

43 objective lens

51 nosepiece (mounting device)

51 a attachment position

53 objective lens

55B mounting adapter

100 microscope system

1. A microscope system comprising: a microscope main unit that generatesillumination light to be radiated onto a specimen and that detectsdetection light from the specimen; and a plurality ofattachable/detachable objective lens units that are selectively attachedto the microscope main unit, the microscope main unit including araising mechanism that can move the attached objective lens unit in anoptical axis direction, wherein the plurality of objective lens unitshave a mounting device that can be attached to the raising mechanism andan objective lens that is mounted on the mounting device in anattachable/detachable manner, and that collects the detection light fromthe specimen; and distances from a mounting position in the microscopemain unit for the mounting device to focal positions of the objectivelenses are set to be mutually equal among these objective lens units. 2.A microscope system according to claim 1, wherein the mounting device ofone of the objective lens units has a mounting device main unit to whichthe objective lens is mounted and a mounting adaptor that is fixed tothe mounting device main unit and that attaches the mounting device mainunit to the microscope main unit in an attachable/detachable manner. 3.A microscope system according to claim 1, wherein the objective lensunit includes a first objective lens and a second objective lens,wherein a distance from an end on a mounting device side of the firstobjective lens to a focal position of the first objective lens in theoptical axis direction is set to be shorter than a distance from an endon a mounting device side of the second objective lens to a focalposition of the second objective lens in the optical axis direction; anda distance in the optical axis direction from a mounting position forthe first objective lens in the mounting device to the focal position ofthe first objective lens is set to be equal to a distance in the opticalaxis direction from the mounting position for the second objective lensin the mounting device to the focal position of the second objectivelens.
 4. A microscope system according to claim 3, further comprising:an objective lens adaptor interposed between the second objective lensand the mounting device.